Goat Thyroxine(T4) ELISA Kit

文獻引用 (2) 說明書下載

產品詳情

產品描述：

山羊甲狀腺素(T4)酶聯免疫試劑盒（CSB-E13384G）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的T4含量。試劑盒檢測范圍為20 ng/mL-320 ng/mL，靈敏度為20 ng/mL。適用于科研領域中對山羊或其他偶蹄目動物甲狀腺功能的動態監測，例如在營養代謝研究、環境內分泌干擾物評估、激素替代治療模型構建中提供定量數據支持，亦可應用于畜牧學科研中飼料添加劑對甲狀腺軸影響的藥效學分析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

T4
種屬：

Capra hircus (Goat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

20 ng/mL-320 ng/mL
靈敏度：

20 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat T4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-101
1:2	Average %	86
	Range %	81-98
1:4	Average %	95
	Range %	90-100
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of goat T4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-110
EDTA plasma (n=4)	95	90-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

最新研究進展：

T4是一種重要的代謝激素，最新研究表明T4與身體免疫系統密切相關。研究人員正在探索T4對免疫細胞功能的影響，以及利用T4來治療免疫系統相關疾病的可能性。