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Fish lysozyme (renal amyloidosis) (LZM) ELISA kit

  • 中文名稱:
    魚溶菌酶(腎淀粉樣變)(LZM)酶聯免疫試劑盒
  • 貨號:
    CSB-E17296Fh
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    魚溶菌酶(腎淀粉樣變)(LZM)酶聯免疫試劑盒(CSB-E17296Fh)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的LZM含量。LZM即溶菌酶,是一種能水解致病菌中黏多糖的堿性酶。廣泛存在于動植物及微生物中,具有抗菌、抗病毒等特性。研究機制主要是通過破壞細菌細胞壁的肽聚糖,使細菌溶解死亡,在醫藥、食品等領域有潛在應用價值。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL,該產品主要應用于水產養殖中的魚類健康監測、免疫相關疾病機制研究以及水質污染對魚類生理影響的評估等領域,為實驗室開展魚類病理學、毒理學及免疫學研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    LZM
  • 種屬:
    Fish
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    3.12 ng/mL-200 ng/mL
  • 靈敏度:
    1.56 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish LZM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:20 Average % 93  
    Range % 85-100  
    1:40 Average % 91  
    Range % 86-99  
    1:80 Average % 95  
    Range % 90-103  
    1:160 Average % 95  
    Range % 90-98  
  • 回收率:
    The recovery of fish LZM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 87-94  
    EDTA plasma (n=4) 93 85-100  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    200 0.088 0.090 0.089    
    100 0.206 0.194 0.200    
    50 0.307 0.300 0.304    
    25 0.418 0.423 0.421    
    12.5 0.627 0.605 0.616    
    6.25 1.045 1.031 1.038    
    3.12 1.648 1.524 1.586    
    0 2.685 2.657 2.671    
  • 數據處理:
  • 貨期:
    3-5 working days

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