Fish hepatocyte growth factor(HGF)ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

魚肝細(xì)胞生長因子(HGF)酶聯(lián)免疫試劑盒（CSB-E16527Fh）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的HGF含量。HGF（肝細(xì)胞生長因子）是一種多功能細(xì)胞因子，主要來源于肝臟的Kupffer細(xì)胞、內(nèi)皮細(xì)胞等，能刺激肝細(xì)胞增殖，并作用于上皮細(xì)胞、造血細(xì)胞、血管內(nèi)皮細(xì)胞等多種細(xì)胞。HGF通過旁分泌或自分泌機(jī)制，在胚胎發(fā)生、創(chuàng)傷愈合、血管發(fā)生、組織器官再生等方面發(fā)揮重要作用。試劑盒檢測范圍為200 pg/mL-8000 pg/mL，適用于基礎(chǔ)醫(yī)學(xué)研究領(lǐng)域，例如肝臟疾病模型中的HGF動態(tài)監(jiān)測、藥物干預(yù)效果評價(jià)或腫瘤微環(huán)境相關(guān)研究，也可用于細(xì)胞培養(yǎng)上清液中HGF分泌水平的定量分析，為探索HGF在病理生理過程中的調(diào)控機(jī)制提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

HGF
Uniprot No.：

F1REK1
種屬：

Fish
樣本類型：

serum, plasma
檢測范圍：

230 pg/mL-8000 pg/mL
靈敏度：

145 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish HGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-105
1:2	Average %	89
	Range %	85-100
1:4	Average %	89
	Range %	82-100
1:8	Average %	93
	Range %	87-99

回收率：

The recovery of fish HGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-98
EDTA plasma (n=4)	95	89-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.