Fish Insulin-like growth factor II(IGF2) ELISA kit

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產(chǎn)品詳情

產(chǎn)品描述：

魚胰島素樣生長因子II(IGF2)酶聯(lián)免疫試劑盒（CSB-EL011088FI）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的IGF2含量。IGF2即胰島素樣生長因子2，是一種多功能細(xì)胞增殖調(diào)控因子，在胚胎生長發(fā)育中起重要作用。其研究機(jī)制主要圍繞與受體結(jié)合激活下游信號通路，影響細(xì)胞的增殖、分化和存活，異常表達(dá)與多種腫瘤的發(fā)生、發(fā)展密切相關(guān)。試劑盒檢測范圍為62.5 pg/mL-1000 pg/mL，本試劑盒適用于水產(chǎn)養(yǎng)殖研究、魚類生理學(xué)實驗、生長調(diào)控機(jī)制探究等科研領(lǐng)域，特別適用于評估飼料營養(yǎng)配比、環(huán)境脅迫對魚類生長的影響或基因工程模型的生物標(biāo)志物分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

igf2Insulin-like growth factor II ELISA Kit; IGF-II ELISA Kit; Erythrotropin ELISA Kit
縮寫：

IGF2
Uniprot No.：

Q02816
種屬：

Fish
樣本類型：

serum, plasma
檢測范圍：

62.5 pg/mL-1000 pg/mL
靈敏度：

31.25 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	87-102
1:200	Average %	95
	Range %	91-99
1:400	Average %	96
	Range %	88-106
1:800	Average %	92
	Range %	88-96

回收率：

The recovery of fish IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	93	85-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. Acts as a ligand for integrin which is required for IGF2 signaling.
亞細(xì)胞定位：

Secreted.
蛋白家族：

Insulin family
數(shù)據(jù)庫鏈接：

UniGene: Omy.11849