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  4. Escherichia coli protein (E.coli P) ELISA kit

Escherichia coli protein (E.coli P) ELISA kit

  • 中文名稱：
    大腸桿菌宿主殘留蛋白(E.coli P)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E11306o
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大腸桿菌宿主殘留蛋白(E.coli P)酶聯(lián)免疫試劑盒（CSB-E11306o）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的E.coli P含量。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，可有效監(jiān)測(cè)生物制品生產(chǎn)過(guò)程中的宿主蛋白殘留；滿足生物制藥工藝開(kāi)發(fā)、重組蛋白純化效果驗(yàn)證、轉(zhuǎn)基因生物樣本分析等科研場(chǎng)景；適用于實(shí)驗(yàn)室規(guī)模的生產(chǎn)監(jiān)控、生物工程研究中的質(zhì)量評(píng)估以及基礎(chǔ)科研中的宿主蛋白殘留分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    E.coli P
  • 種屬：
    Escherichia Coli
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Microbiology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of escherichia coli protein in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 87  
    Range % 82-93  
    1:2 Average % 94  
    Range % 89-99  
    1:4 Average % 92  
    Range % 87-96  
    1:8 Average % 95  
    Range % 90-100  
  • 回收率：
    The recovery of escherichia coli protein spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-97  
    EDTA plasma (n=4) 96 89-103  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.048 2.111 2.080 1.929  
    5 1.769 1.810 1.790 1.639  
    2.5 1.236 1.314 1.275 1.124  
    1.25 0.752 0.763 0.758 0.607  
    0.625 0.419 0.424 0.422 0.271  
    0.312 0.306 0.315 0.311 0.160  
    0.156 0.237 0.241 0.239 0.088  
    0 0.150 0.152 0.151 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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