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Dog β2-microglobulin,BMG/β2-MG ELISA Kit

  • 中文名稱:
    犬β2微球蛋白(BMG/β2-MG)酶聯免疫試劑盒
  • 貨號:
    CSB-E13768c
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    犬β2微球蛋白(BMG/β2-MG)酶聯免疫試劑盒(CSB-E13768c)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的B2M含量。B2M全稱為β2微球蛋白,其在免疫識別、抗原呈遞等過程中發揮重要作用。它與主要組織相容性復合體Ⅰ類分子結合,參與機體免疫監視。研究發現其表達異常與腫瘤、自身免疫病等多種疾病相關,目前研究聚焦于其在疾病發生發展機制及作為治療靶點的潛力。試劑盒檢測范圍為0.78 μg/mL-50 μg/mL,適用于科研場景下犬類腎臟功能評估、免疫相關疾病機制研究或藥物毒性實驗中生物標志物的檢測,為動物實驗提供可靠的數據支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    B2M ELISA Kit; Beta-2-microglobulin ELISA Kit; Fragment ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.78 μg/mL-50 μg/mL
  • 靈敏度:
    0.2 μg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of dog β2-MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 90
    Range % 83-99
    1:2 Average % 92
    Range % 85-100
    1:4 Average % 95
    Range % 85-105
    1:8 Average % 95
    Range % 88-102
  • 回收率:
    The recovery of dog β2-MG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 86-99
    EDTA plasma (n=4) 95 91-100
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average Corrected
    50 2.554 2.569 2.562 2.430
    25 2.272 2.256 2.264 2.132
    12.5 1.792 1.803 1.798 1.666
    6.25 1.153 1.180 1.167 1.035
    3.13 0.625 0.616 0.621 0.489
    1.56 0.363 0.379 0.371 0.239
    0.78 0.261 0.264 0.263 0.131
    0 0.135 0.129 0.132  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
  • 亞細胞定位:
    Secreted.
  • 數據庫鏈接:

    UniGene: Cfa.29179



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