Dog Myeloperoxidase (MPO) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬髓過氧化物酶(MPO)酶聯(lián)免疫試劑盒（CSB-E12762c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MPO含量。MPO（髓過氧化物酶）是一種存在于中性粒細胞和單核細胞中的血紅素蛋白。它在炎癥反應(yīng)中起關(guān)鍵作用，催化過氧化氫與氯離子反應(yīng)生成次氯酸等強氧化劑。其異常表達與心血管疾病、神經(jīng)退行性疾病等多種病理過程相關(guān)，研究其機制有助于開發(fā)針對性治療策略。試劑盒檢測范圍為31.25 ng/mL-2000 ng/mL，科研人員可將其應(yīng)用于犬類炎癥性疾病模型構(gòu)建、藥物抗炎效果評估、免疫調(diào)控機制研究等領(lǐng)域，為獸醫(yī)病理學(xué)、比較醫(yī)學(xué)及動物模型研究提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

MPO
Uniprot No.：

F1PQ52
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

31.25 ng/mL-2000 ng/mL
靈敏度：

7.81 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-94
1:2	Average %	101
	Range %	97-106
1:4	Average %	90
	Range %	87-95
1:8	Average %	91
	Range %	87-98

回收率：

The recovery of dog MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-95
EDTA plasma (n=4)	91	87-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.