Dog Complement 4(C4) ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬補(bǔ)體蛋白4(C4)酶聯(lián)免疫試劑盒（CSB-EQ027970DO）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的C4含量。試劑盒檢測(cè)范圍為31.25 ng/mL-2000 ng/mL，適用于動(dòng)物模型研究、免疫系統(tǒng)功能評(píng)估、藥物干預(yù)效果觀測(cè)等科研場(chǎng)景，為犬類基礎(chǔ)醫(yī)學(xué)研究提供標(biāo)準(zhǔn)化工具；其濃度變化可為研究犬類免疫相關(guān)疾病機(jī)制、感染性疾病進(jìn)程或自身免疫異常提供重要依據(jù)本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
縮寫：

C4
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

31.25 ng/mL-2000 ng/mL
靈敏度：

7.8 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	97
	Range %	92-105
1:400	Average %	96
	Range %	92-99
1:800	Average %	103
	Range %	98-109
1:1600	Average %	87
	Range %	82-91

回收率：

The recovery of dog C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-98
EDTA plasma (n=4)	90	87-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.