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Deer Insulin-like growth factors 1,IGF-1 ELISA Kit

  • 中文名稱:
    鹿胰島素樣生長因子1(IGF-1)酶聯免疫試劑盒
  • 貨號:
    CSB-E12644De
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    鹿胰島素樣生長因子1(IGF-1)酶聯免疫試劑盒(CSB-E12644De)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的IGF-1含量。IGF-1(胰島素樣生長因子-1)是一種由IGF1基因編碼的蛋白質,與胰島素具有相似的結構和功能。它主要在肝臟合成,并通過生長激素(GH)的調節促進生長和發育。IGF-1通過激活IGF-1受體,在多個組織和器官中調節細胞增殖、分化和凋亡。其研究機制涉及IGF-1與受體的結合,以及通過多種信號通路(如PI3K/AKT和MEK/ERK)影響細胞功能。試劑盒檢測范圍為1 ng/mL-200 ng/mL,科研人員可將其應用于鹿科動物生長發育研究、營養干預實驗、繁殖生物學分析以及代謝相關疾病模型的機制探索,例如評估不同飼養條件下IGF - 1的動態變化,或探究其在組織再生修復中的作用。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    IGF-1
  • Uniprot No.:
  • 種屬:
    Deer
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    1 ng/mL-200 ng/mL
  • 靈敏度:
    0.5 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of deer IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %98
    Range %92-104
    1:2Average %95
    Range %89-104
    1:4Average %99
    Range %87-107
    1:8Average %96
    Range %90-102
  • 回收率:
    The recovery of deer IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9690-102
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    2000.175 0.174 0.175
    500.333 0.316 0.325
    160.622 0.640 0.631
    41.040 1.048 1.044
    11.416 1.389 1.403
    02.577 2.633 2.605
  • 數據處理:
  • 貨期:
    3-5 working days

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