Chicken Parathyroid hormone,PTH ELISA Kit

產品詳情

文獻引用 (7) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

雞甲狀旁腺激素(PTH)酶聯免疫試劑盒（CSB-E11880Ch）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PTH含量。PTH即甲狀旁腺激素，它主要由甲狀旁腺主細胞合成和分泌，在調節鈣磷代謝、維持血鈣平衡上意義重大。研究發現PTH能通過與受體結合激活信號通路，促進骨代謝及腎臟對鈣的重吸收等，其相關機制研究對骨病等治療有重要潛在價值。試劑盒檢測范圍為6.25 pg/mL-100 pg/mL，該產品廣泛應用于禽類鈣代謝機制研究、飼料營養評估、疾病模型構建等科研場景，尤其適用于探究環境因素、遺傳變異或藥物干預對禽類甲狀旁腺功能的影響。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

PTH ELISA Kit; Parathyroid hormone ELISA Kit; PTH ELISA Kit
縮寫：

PTH
Uniprot No.：

P15743
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 pg/mL-100 pg/mL
靈敏度：

6.25 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-95
1:2	Average %	96
	Range %	92-103
1:4	Average %	85
	Range %	81-86
1:8	Average %	90
	Range %	83-98

回收率：

The recovery of chicken PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-98
EDTA plasma (n=4)	94	90-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion.
基因功能參考文獻：
1. A single nucleotide polymorphism in parathyroid hormone affected the eggshell percentage and breaking strength, and it may be associated with the variation of serum calcium and PTH level. PMID: 20852100
亞細胞定位：

Secreted.
蛋白家族：

Parathyroid hormone family
數據庫鏈接：

KEGG: gga:396436

STRING: 9031.ENSGALP00000008579

UniGene: Gga.78