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Chicken Interleukin 17,IL-17 ELISA Kit

  • 中文名稱:
    雞白介素17(IL-17)酶聯免疫試劑盒
  • 貨號:
    CSB-E04607Ch
  • 規格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    雞白介素17(IL-17)酶聯免疫試劑盒(CSB-E04607Ch)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的IL17含量。IL-17是一種由Th17細胞分泌的促炎細胞因子,參與調節免疫反應和炎癥過程。其在銀屑病、類風濕關節炎等自身免疫疾病中起重要作用,同時也是腫瘤發生和進展的關鍵因子。研究IL-17機制有助于開發針對相關疾病的治療方法。試劑盒檢測范圍為150 pg/mL-6000 pg/mL,適用于家禽免疫學研究領域,如探究病原體感染后宿主免疫應答動態、評估疫苗免疫效果或解析炎癥性疾病模型中的細胞因子調控網絡,為禽類基礎免疫機制研究及疾病防控提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    IL-17
  • Uniprot No.:
  • 種屬:
    Gallus gallus (Chicken)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    150 pg/mL-6000 pg/mL
  • 靈敏度:
    150 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 82-100  
    1:2 Average % 95  
    Range % 90-100  
    1:4 Average % 96  
    Range % 90-101  
    1:8 Average % 91  
    Range % 85-98  
  • 回收率:
    The recovery of chicken IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 85-96  
    EDTA plasma (n=4) 92 86-98  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    0 1.914 2.027 1.971    
    150 1.306 1.329 1.318    
    450 1.039 1.102 1.071    
    1380 0.755 0.702 0.729    
    3000 0.484 0.462 0.473    
    6000 0.351 0.348 0.350    
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價



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