Canine Parathyroid hormone(PTH) ELISA Kit

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產品詳情

產品描述：

犬甲狀旁腺激素(PTH)酶聯免疫試劑盒（CSB-E17575c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PTH含量。甲狀旁腺激素（PTH）是甲狀旁腺主細胞分泌的肽類激素。其可調節鈣、磷代謝，維持血鈣水平穩定。在研究機制上，PTH作用于骨骼、腎臟等靶器官，通過與受體結合激活信號通路，促進骨鈣釋放、腎小管對鈣重吸收及磷排泄等。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于探索犬鈣代謝異常、甲狀旁腺相關疾病機制及內分泌調控研究；為科研領域提供高效工具，支持動物模型研究、營養代謝實驗及疾病機制探索本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

PTH ELISA Kit; Parathyroid hormone ELISA Kit; PTH ELISA Kit; Parathyrin ELISA Kit
縮寫：

PTH
Uniprot No.：

P52212
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	86-102
1:2	Average %	96
	Range %	90-103
1:4	Average %	89
	Range %	83-102
1:8	Average %	97
	Range %	92-105

回收率：

The recovery of caninePTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	82-105
EDTA plasma (n=4)	96	90-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
基因功能參考文獻：
1. Acute metabolic and respiratory alkalosis markedly decreased PTH values during normocalcemia and delayed the PTH response to hypocalcemia PMID: 12929937
亞細胞定位：

Secreted.
蛋白家族：

Parathyroid hormone family
數據庫鏈接：

KEGG: cfa:403986

STRING: 9615.ENSCAFP00000012029

UniGene: Cfa.3855