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Canine Matrix metalloproteinase 13,MMP-13 ELISA kit

  • 中文名稱:
    犬基質金屬蛋白酶13(MMP-13)酶聯免疫試劑盒
  • 貨號:
    CSB-E14055c
  • 規格:
    96T/48T
  • 價格:
    ¥3900/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    犬基質金屬蛋白酶13(MMP-13)酶聯免疫試劑盒(CSB-E14055c)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的MMP-13含量。MMP-13即基質金屬蛋白酶13,在人體組織重塑、炎癥反應等生理過程中起重要作用。異常表達與多種病理情況相關,如關節炎、癌癥等。研究主要圍繞其酶活性調控機制,旨在開發針對此靶點的藥物,以干預相關疾病進展。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL,適用于科研領域對犬類骨關節疾病模型、軟骨退行性病變機制以及抗炎藥物研發等研究方向的樣本檢測需求;滿足實驗室對犬MMP - 13蛋白表達水平的高通量檢測及動態監測要求。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    MMP-13
  • Uniprot No.:
  • 種屬:
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍:
    0.156 ng/mL-10 ng/mL
  • 靈敏度:
    0.039 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cancer
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %93
    Range %88-99
    1:2Average %99
    Range %95-106
    1:4Average %87
    Range %84-92
    1:8Average %91
    Range %85-98
  • 回收率:
    The recovery of canine MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9187-95
    EDTA plasma (n=4)8885-92
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    103.160 3.255 3.208 3.107
    52.614 2.712 2.663 2.562
    2.51.784 1.932 1.858 1.757
    1.251.026 1.030 1.028 0.927
    0.6250.478 0.487 0.483 0.382
    0.3120.264 0.256 0.260 0.159
    0.1560.156 0.166 0.161 0.060
    00.105 0.097 0.101
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價



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