Bovine gonadotropin-releasing hormone(GnRH)ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

牛促性腺激素釋放激素(GnRH)酶聯(lián)免疫試劑盒（CSB-E15857B）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的GnRH含量。GnRH（促性腺激素釋放激素）是一種由下丘腦分泌的激素，主要調(diào)節(jié)生殖系統(tǒng)的功能。其研究背景涉及生殖調(diào)控、內(nèi)分泌系統(tǒng)以及腫瘤治療等領(lǐng)域。GnRH通過(guò)作用于垂體前葉，調(diào)節(jié)促性腺激素的分泌，進(jìn)而影響性腺發(fā)育和生殖過(guò)程。研究機(jī)制主要圍繞GnRH的受體、信號(hào)通路及其在不同疾病中的作用展開(kāi)。試劑盒檢測(cè)范圍為100 pg/mL-4000 pg/mL，適用于研究牛生殖周期調(diào)控、繁殖力評(píng)估、激素分泌動(dòng)態(tài)監(jiān)測(cè)等科研場(chǎng)景，特別適用于畜牧業(yè)中生殖內(nèi)分泌機(jī)制探索及生殖健康相關(guān)研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

GnRH
Uniprot No.：

Q0VBW7
種屬：

Bos taurus (Bovine)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	83-95
1:2	Average %	95
	Range %	91-101
1:4	Average %	95
	Range %	89-101
1:8	Average %	90
	Range %	85-98

回收率：

The recovery of bovine GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	97	94-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.