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Bovine Vitamin A (VA) ELISA Kit

  • 中文名稱:
    牛維生素A(VA)酶聯免疫試劑盒
  • 貨號:
    CSB-E17987B
  • 規格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    牛維生素A(VA)酶聯免疫試劑盒(CSB-E17987B)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的VA含量。試劑盒檢測范圍為6.25 ng/mL-100 ng/mL,靈敏度為4.672 ng/mL。本試劑盒適用于科研場景中牛類樣本的維生素A水平分析,例如探究飼料營養干預對VA吸收的影響、疾病狀態下VA代謝變化或組織分布特征等方向本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    VA
  • 種屬:
    Bos taurus (Bovine)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    6.25 ng/mL-100 ng/mL
  • 靈敏度:
    4.672 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Metabolism
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine VA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:10 Average % 95  
    Range % 91-99  
    1:20 Average % 103  
    Range % 99-107  
    1:40 Average % 98  
    Range % 93-103  
    1:80 Average % 92  
    Range % 89-95  
  • 回收率:
    The recovery of bovine VA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 93-104  
    EDTA plasma (n=4) 89 83-95  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    100 0.098 0.095 0.097    
    50 0.150 0.163 0.157    
    25 0.263 0.268 0.266    
    12.5 0.551 0.565 0.558    
    6.25 1.037 1.065 1.051    
    0 2.266 2.287 2.277    
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 最新研究進展:
    最新研究表明,VA蛋白在細胞分化、免疫應答和炎癥反應等生物學過程中起著重要的作用。


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