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Bovine Thrombin-antithrombin III Complex, TAT-III ELISA Kit

  • 中文名稱:
    牛凝血酶抗凝血酶Ⅲ復合物(TAT-III)酶聯免疫試劑盒
  • 貨號:
    CSB-E14133B
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    牛凝血酶抗凝血酶Ⅲ復合物(TAT-III)酶聯免疫試劑盒(CSB-E14133B)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的TAT-III含量。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL,本試劑盒可應用于心血管疾病動物模型研究、凝血相關藥物開發中的藥效評價,或探索凝血 - 抗凝系統動態平衡的分子調控機制,為科研領域提供靈敏、特異的檢測方案。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    TAT-III
  • 種屬:
    Bos taurus (Bovine)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    1.56 pg/mL-100 pg/mL
  • 靈敏度:
    0.78 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cardiovascular
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine TAT-III in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:20 Average % 92    
    Range % 88-96    
    1:40 Average % 85    
    Range % 80-90    
    1:80 Average % 104    
    Range % 100-110    
    1:160 Average % 97    
    Range % 92-101    
  • 回收率:
    The recovery of bovine TAT-III spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 90-100    
    EDTA plasma (n=4) 89 85-93    
                   
                   
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average      
    100 0.159 0.163 0.161      
    50 0.279 0.267 0.273      
    25 0.455 0.471 0.463      
    12.5 0.724 0.704 0.714      
    6.25 1.071 1.006 1.039      
    3.12 1.279 1.322 1.301      
    1.56 1.683 1.718 1.701      
    0 2.106 2.235 2.171      
  • 數據處理:
  • 貨期:
    3-5 working days

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