Bovine IgE ELISA Kit

產品詳情

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說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

牛免疫球蛋白E （IgE）酶聯免疫試劑盒（CSB-E13028B）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的IgE含量。IgE即免疫球蛋白E，是介導I型超敏反應的抗體。在許多過敏性疾病如哮喘、過敏性鼻炎中，IgE水平常異常升高。其研究機制主要圍繞抑制IgE生成、阻斷IgE與受體結合等方面，通過這些手段有望控制過敏癥狀、改善相關疾病病情。試劑盒檢測范圍為62.5 ng/mL-4000 ng/mL，適用于科研領域對牛過敏反應機制、寄生蟲感染免疫應答及免疫調控相關研究；研究者可通過該產品監測實驗動物模型中IgE的動態變化，評估免疫干預效果，或用于畜牧領域牛群免疫健康狀態的基礎研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IgE
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma
檢測范圍：

62.5 ng/mL-4000 ng/mL
靈敏度：

15.6 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	83-102
1:2	Average %	93
	Range %	88-99
1:4	Average %	94
	Range %	90-102
1:8	Average %	90
	Range %	81-99

回收率：

The recovery of bovine IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-104
EDTA plasma (n=4)	95	87-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.