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Bovine Caspase 7(Casp-7) ELISA Kit

  • 中文名稱:
    牛胱天蛋白酶7(Casp-7)酶聯免疫試劑盒
  • 貨號:
    CSB-E17315B
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    牛胱天蛋白酶7(Casp-7)酶聯免疫試劑盒(CSB-E17315B)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的Casp7含量。Casp7(Caspase-7)是一種參與細胞凋亡過程的半胱氨酸型內肽酶,屬于caspase家族。它在細胞凋亡的執行階段發揮關鍵作用,通過切割下游底物如PARP來促進程序性細胞死亡。Casp7的表達和活性與多種疾病如癌癥、阿爾茨海默病等密切相關。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL,該產品可用于科研領域細胞凋亡機制研究、藥物或毒素誘導的凋亡效應評估、免疫相關疾病模型分析等場景,例如探究病原感染、腫瘤治療或神經系統疾病中的Casp - 7表達變化。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Bos taurus (Bovine)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.625 ng/mL-40 ng/mL
  • 靈敏度:
    0.156 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cell Biology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine Casp-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %95
    Range %85-101
    1:2Average %95
    Range %88-102
    1:4Average %89
    Range %83-100
    1:8Average %93
    Range %89-106
  • 回收率:
    The recovery of bovine Casp-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9790-112
    EDTA plasma (n=4)9384-99
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.546 2.457 2.502 2.369
    201.883 1.905 1.894 1.761
    101.136 1.124 1.130 0.997
    50.726 0.711 0.719 0.586
    2.50.407 0.397 0.402 0.269
    1.250.305 0.313 0.309 0.176
    0.6250.196 0.204 0.200 0.067
    00.130 0.136 0.133
  • 數據處理:
  • 貨期:
    3-5 working days

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